Services Available at the RCMI Translational Proteomics Center

  1. Protein extraction and purification. We provide training and suggestions regarding protein extraction and preparation from a specific biological specimen or experimental sample (different types of tissue, blood, urine, cerebrospinal fluid, tissue culture, cells suspensions, purified and recombinant proteins in solution) in consonance to the proteomic approach the investigator would like to pursue. Please consult to the Key Director for specifics recommendations. This service is provided with direct collaboration from Dr. Horacio Serrano.
  2. Protein separation. Sample separation technique such as one- (1D) gel electrophoresis is available at the Center.
  3. Immunoprecipitation. We offer the service of immunoprecipitation of your protein of interest and evaluate possible protein-protein interactions.
  4. Sample Processing for Mass Spectrometry. In gel-digestion using Trypsin and peptide extractions are services available at the Center for further sample processing, to either identify or relatively quantify proteins.
  5. Protein identification. A top notch Easy-nano LC 1000 couple to LTQ XL mass spectrometer from Thermo Scientific is available and configured for maximum coverage in protein identification.
  6. Quantitative Proteomics. We are currently in the process of incorporating a new Q-Exactive Plus instrumentation for the analysis of tagged samples and other quantitative proteomics methodologies. Available techniques to be used for this instrument: Isobaric Tag for Relative and Absolute Quantitation (iTRAQ), Tandem Mass Tag (TMT) System, and Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC). RCMI Bioinformatics support is recommended for completion of the quantitative analysis.
  7. Post-translational modification analysis. In our interest to provide methods to study post-translational modifications we are in the process of incorporating phosphoproteomics analysis by mass spectrometry.
  8. Validation Analyses. The Center provides equipment as well as the services for western blotting, ELISA, fluorometric and luminometric detection technologies for direct protein expression validations. Investigators are responsible for supporting funds for specific antibodies and reagents.